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Monoclonal Antibodies to the Human γ 2 Subunit of the GABA A /Benzodiazepine Receptors
Author(s) -
Fernando Lawrence P.,
Khan Zafar U.,
McKernan Ruth M.,
De Blas Angel L.
Publication year - 1995
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1995.64031305.x
Subject(s) - monoclonal antibody , receptor , gabaa receptor , fusion protein , microbiology and biotechnology , protein subunit , cys loop receptors , biochemistry , immunoprecipitation , biology , antibody , chemistry , recombinant dna , immunology , acetylcholine receptor , gene , nicotinic acetylcholine receptor
The large intracellular loop (IL) of the γ 2 subunit of the cloned human γ‐aminobutyric acid A (GABA A ) receptor (γ 2 IL) was expressed in bacteria as glutathione‐ S ‐transferase and staphylococcal protein A fusion proteins. Mice were immunized with the fusion proteins (one protein per animal), and monoclonal antibodies were obtained. Six monoclonal antibodies reacted with the γ 2 IL moiety of the fusion proteins. Three of these monoclonal antibodies also immunoprecipitated a high proportion of the GABA A /benzodiazepine receptors from bovine and rat brain and reacted with a wide 44,000–49,000‐M r peptide band in immunoblots of affinity‐purified GABA A receptors. These monoclonal antibodies are valuable reagents for the molecular characterization of the GABA A receptors in various brain regions.