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[ 3 H]Nemonapride and [ 3 H]Spiperone Label Equivalent Numbers of D 2 and D 3 Dopamine Receptors in a Range of Tissues and Under Different Conditions
Author(s) -
Vile John M.,
D'Souza Ursula M.,
Strange Philip G.
Publication year - 1995
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1995.64020940.x
Subject(s) - spiperone , dopamine , chemistry , receptor , dopamine receptor , radiochemistry , endocrinology , biology , biochemistry
[ 3 H]Nemonapride and [ 3 H]spiperone are very widely used to study dopaminergic systems in vitro and in vivo, but it has been reported that [ 3 H]nemonapride and [ 3 H]spiperone give markedly different B max values for preparations of D 2 dopamine receptors from recombinant cell lines or animal tissues. We have used the two radioligands in parallel to study a range of dopamine receptors [D 2(short) , D 2(long) , and D 3 ] in different buffers. B max values derived using either radioligand differ by an average of <20%, independent of receptor type or buffer conditions. All competition experiments show that the two ligands compete at a single site. It seems that [ 3 H]spiperone and [ 3 H]nemonapride do not differentiate between different forms or populations of D 2 ‐like receptors.