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The Simian Virus 40 Large T Antigen Does Not Inhibit Translation of the 14‐kDa Myelin Basic Protein mRNA in Reticulocyte Lysates or in Transfected Cells
Author(s) -
Ueno S.,
Foster L.,
Hifumi G. T.,
Tennekoon G. I.,
Campagi A. T.
Publication year - 1995
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1995.64020928.x
Subject(s) - myelin basic protein , microbiology and biotechnology , transfection , reticulocyte , biology , messenger rna , complementary dna , oligodendrocyte , sv40 large t antigen , cell culture , myelin , gene , biochemistry , genetics , neuroscience , central nervous system
Viral T antigens are transcription factors that have been suspected of inhibiting expression of the myelin basic protein (MBP) mRNA at the translational level in vitro and in vivo. The effect of simian virus 40 (SV40) large T antigen (T‐ag) was examined on the translation of the 14‐kDa MBP mRNA in reticulocyte lysates and on MBP expression after transfection into cells that express SV40 T‐ag. SV40 T‐ag did not inhibit translation of 14‐kDa MBP cRNAs in cell‐free translations even at 30 µ M (∼600 µg/ml) T‐ag. Permanent transfection of COS‐1 cells (which endogenously express SV40 T‐ag) with the 14‐kDa MBP cDNA resulted in the expression of the 14‐kDa MBP as determined by western blot analysis. Permanent transfection of N20.1 cells, an oligodendrocyte line immortalized with a temperature‐sensitive SV40 T‐ag, with the 14‐kDa MBP cDNA construct also resulted in the expression of the 14‐kDa MBP under conditions in which the cells expressed functional SV40 T‐ag. These results indicate that SV40 T‐ag does not prevent expression of the MBP gene at the translational level and that in those immortalized oligodendrocyte lines that express MBP mRNA, but not MBP protein, some factor other than the SV40 large T‐ag is responsible for the posttranscriptional regulation.