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High Resolution Proton NMR Spectroscopy of Multiple Sclerosis Lesions
Author(s) -
Davies Siân E. C.,
Newcombe Jia,
Williams Stephen R.,
McDonald W. Ian,
Clark John B.
Publication year - 1995
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1995.64020742.x
Subject(s) - creatine , choline , white matter , chemistry , metabolite , multiple sclerosis , in vivo , nuclear magnetic resonance spectroscopy , endocrinology , medicine , nuclear magnetic resonance , magnetic resonance imaging , biochemistry , biology , stereochemistry , physics , microbiology and biotechnology , psychiatry , radiology
Tissue from postmortem multiple sclerosis and normal control brains was extracted with perchloric acid and analysed using proton NMR spectroscopy. The content of N ‐acetyl‐derived groups (the sum of N ‐acetylaspartate, acetate, and N ‐acetylaspartylglutamate) was decreased in multiple sclerosis plaques compared with normal control white matter (mean, 4.36 vs. 6.64 µmol/g wet weight). In normal appearing white matter adjacent to plaques a corresponding decrease was seen, with no change in white matter distant from plaques. A decrease in the content of total creatine was observed in multiple sclerosis plaques in comparison with normal control white matter (mean, 4.64 vs. 6.56 µmol/g wet weight), which correlated strongly with the decrease in N ‐acetyl‐derived groups. No changes in other metabolites such as total choline or myo ‐inositol were seen. The decreases in content of N ‐acetyl‐derived groups are in agreement with observations from in vivo proton NMR spectroscopy in multiple sclerosis patients. The decrease in total creatine is in contrast to most of the observations made in vivo where total creatine is assumed to be unchanged and metabolite levels are often expressed as a total creatine ratio. The use of a total creatine ratio in vivo could lead to an underestimation of reductions in N ‐acetylaspartate and an apparent increase in other metabolites in the multiple sclerosis lesion.