Premium
Identification and Characterization of Various Cholecystokinin B Receptor mRNA Forms in Rat Brain Tissue and Partial Determination of the Cholecystokinin B Receptor Gene Structure
Author(s) -
Jagerschmidt A.,
Popovici T.,
O'Donohue M.,
Roques B. P.
Publication year - 1994
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1994.63041199.x
Subject(s) - cholecystokinin , messenger rna , biology , microbiology and biotechnology , cerebellum , cholecystokinin receptor , receptor , gene expression , cholecystokinin b receptor , hippocampus , precursor mrna , gene , biochemistry , endocrinology , rna , rna splicing
Using the powerful method of DNA amplification, we have been able to isolate several cholecystokinin B (CCK B ) receptor (CCK B ‐R) mRNA forms from rat brain tissue, allowing the detection of a truncated mRNA species and the determination of the CCK B ‐R gene structure. Unspliced precursor mRNA and the mature form were identified in the cerebral cortex, hypothalamus, and hippocampus in apparently differing proportions according to the region examined, suggesting that the expression of the CCK B ‐R could be modulated at a posttranscriptional level. In the case of the cerebellum, only a completely unspliced mRNA form was detected, in agreement with previous studies in which CCK B ligand binding sites were not observed. In contrast, a truncated CCK B ‐R mRNA, lacking 250 bp, was detected in all the studied brain regions except for the cerebellum. This mRNA, for which a cellular function has not been assigned, potentially encodes a protein consisting of 168 amino acids.