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Synergistic Activation of DNA Synthesis in Astrocytes by Fibroblast Growth Factors and Extracellular ATP
Author(s) -
Neary J. T.,
Whittemore S. R.,
Zhu Q.,
Norenberg M. D.
Publication year - 1994
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1994.63020490.x
Subject(s) - purinergic receptor , extracellular , dna synthesis , p2y receptor , fibroblast growth factor , growth factor , microbiology and biotechnology , epidermal growth factor , purinergic signalling , adenosine triphosphate , adenosine , biochemistry , biology , astrocyte , intracellular , basic fibroblast growth factor , fibroblast , chemistry , dna , receptor , adenosine receptor , endocrinology , in vitro , agonist , central nervous system
The effects of extracellular ATP and polypeptide growth factors on DNA synthesis in primary cultures of rat astrocytes have been examined. It was found that ATP acts synergistically with either acidic or basic fibroblast growth factor to stimulate DNA synthesis. The specificity of this effect was demonstrated by the inability of ATP to potentiate DNA synthesis induced by platelet‐derived growth factor or epidermal growth factor. ATP appears to act via P 2 purinergic receptors, because (a) it was more effective than adenosine and (b) the synergistic effect was observed with the hydrolysis‐resistant P 2 agonists, ADPβS and ATPγS. The evidence suggests that extracellular ATP may be an important factor in regulating the extent of gliosis and, as such, may be involved in mechanisms of neural injury and repair.