Premium
Primary Structure of the Human Platelet Serotonin 5‐HT 2A Receptor: Identity with Frontal Cortex Serotonin 5‐HT 2A Receptor
Author(s) -
Cook Edwin H.,
Fletcher Kathlyn E.,
Wainwright Mark,
Marks Nancy,
Yan Shuya,
Leventhal Bennett L.
Publication year - 1994
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1994.63020465.x
Subject(s) - serotonin , 5 ht receptor , receptor , biology , 5 ht5a receptor , complementary dna , platelet , human brain , radioligand , endocrinology , medicine , neuroscience , genetics , gene , immunology
Previous radioligand binding studies have demonstrated human platelet serotonin 2A (5‐HT 2A ) receptor binding sites. Pharmacological similarities between platelet and frontal cortex 5‐HT 2A receptor binding parameters have been demonstrated. However, it is not clear whether the platelet 5‐HT 2A receptor primary structure is identical to that of the brain receptor. Three overlapping cDNAs were obtained to span completely the coding region of the 5‐HT 2A receptor. These clones were sequenced with external and internal primers. The nucleotide sequence of human platelet 5‐HT 2A cDNA was identical to that reported for the human frontal cortex 5‐HT 2A receptor, except for nucleotide 102 (T → C), which has been reported to represent a normal DNA polymorphism that does not alter the amino acid sequence. This finding may have implications in the study of neuropsychiatric disorders for which altered platelet 5‐HT 2A receptor binding has been demonstrated.