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Intracerebroventricular Administration of AT 1 Receptor Antisense Oligonucleotides Inhibits the Behavioral Actions of Angiotensin II
Author(s) -
Sakai R. R.,
He P. F.,
Yang X. D.,
Ma L. Y.,
Guo Y. F.,
Reilly J. J.,
Moga C. N.,
Fluharty S. J.
Publication year - 1994
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1994.62052053.x
Subject(s) - oligonucleotide , receptor , angiotensin ii , pharmacology , chemistry , neuroscience , biology , endocrinology , microbiology and biotechnology , biochemistry , dna
Antisense Oligonucleotides were developed to study the expression and function of angiotensin type 1 (AT 1 ) receptors in cultured cells and brain. In both liver epithelial WB and neuro‐blastoma N1E‐115 cells AT 1 antisense oligomers substantially decreased AT 1 receptor density, whereas angiotensin type 2 (AT 2 ) receptors remained unchanged. Similarly, repeated intracerebroventricular injections of AT 1 antisense oligomers in rats decreased AT 1 receptor density in hypothalamic‐thalamic‐septal tissue, and AT 2 receptors were unaffected. Intracerebroventricular antisense oligomers also attenuated drinking elicited by intra‐cerebroventricular angiotensin II but not the cholinomimetic carbachol. Collectively, these results demonstrate that antisense Oligonucleotides attenuate angiotensin receptor expression and function in behaving animals.