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B 1 and B 2 Bradykinin Receptors Encoded by Distinct mRNAs
Author(s) -
Webb Michael,
McIntyre Peter,
Phillips Elsa
Publication year - 1994
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1994.62041247.x
Subject(s) - receptor , biology , microbiology and biotechnology , bradykinin , b cell receptor , messenger rna , genetics , b cell , gene , antibody
Bradykinin receptors have been subdivided into at least two major pharmacological subtypes, B 1 and B 2 . The cDNAs encoding functional B 2 receptors have recently been cloned, but no molecular information exists at present on the B 1 receptor. In this article, we describe experiments examining the possible relationship between the mRNAs encoding the B 1 and B 2 types of receptor. We showed previously that the Human fibroblast cell line W138 expresses both B 1 and B 2 receptors. In this report, we describe oocyte expression experiments showing that the B 1 receptor in W138 human fibroblast cells is encoded by a distinct mRNA ∼2 kb shorter than that encoding the B 2 receptor. We have used an antisense approach in conjunction with the oocyte expression system to demonstrate that the two messages differ in sequence at several locations throughout the length of the B 2 sequence. Taken together with the mixed pharmacology exhibited in some expression systems by the cloned mouse receptor, the data indicate that B 1 ‐type pharmacology may arise from two independent molecular mechanisms.