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Phosphorylation‐Dependent Immunoreactivity of Neurofilaments and the Rate of Slow Axonal Transport in the Central and Peripheral Axons of the Rat Dorsal Root Ganglion
Author(s) -
Archer D. R.,
Watson D. F.,
Griffin J. W.
Publication year - 1994
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1994.62031119.x
Subject(s) - neurofilament , dorsal root ganglion , axoplasmic transport , sciatic nerve , cytoskeleton , tubulin , phosphorylation , microtubule , chemistry , sensory neuron , anatomy , peripheral , biology , microbiology and biotechnology , neuroscience , central nervous system , sensory system , biochemistry , medicine , cell , immunohistochemistry , immunology
The rate of axonal transport of tubulin, actin, and the neurofilament proteins was measured in the peripheral and central projections of the rat L5 dorsal root ganglion (DRG). [ 35 S]Methionine was injected into the DRG, and the “front” of the radiolabeled protein was located 7, 14, and 20 days postinjection. Transport rates calculated for the neurofilament triplet proteins, tubulin, and actin in the peripheral nerve were ∼ 1.5‐fold faster than those in the dorsal root. A progressive decrease in the rate of transport was observed from 7 to 20 days after radiolabeling in both the central and peripheral directions (neurofilaments, ∼ 1.7‐fold; tubulin/actin, 2.1‐fold). A surgical preparation, leaving the peripheral sciatic nerve with predominantly sensory fibers, was the basis for ELISAs for phosphorylation‐dependent immunoreactivity of the high‐molecular‐weight neurofilament protein. In both dorsal roots and peripheral sensory axons the degree of phosphorylation was greater in nerve segments further away from the cell bodies. The degree of phosphorylation‐related immunoreactivity correlates with the slowing of transport of radiolabeled cytoskeletal protein.