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Depolarization and Neurotransmitters Increase Neuronal Protein Tyrosine Phosphorylation
Author(s) -
Siciliano Julio C.,
Gelman Michèle,
Girault JeanAntoine
Publication year - 1994
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1994.62030950.x
Subject(s) - tyrosine phosphorylation , metabotropic glutamate receptor , biology , phosphorylation , metabotropic receptor , protein phosphorylation , microbiology and biotechnology , chemistry , glutamate receptor , biochemistry , protein kinase a , receptor
In rat hippocampal slices and in neurons in primary culture, K + ‐induced depolarization increased markedly and rapidly tyrosine phosphorylation of a 110‐kDa protein (pp110) and, to a lesser degree, of a 120‐kDa protein (pp120), in a calcium‐dependent fashion. Qlutamate, 1‐aminocyclopentane‐ trans ‐1,3‐dicarboxylic acid (an agonist of metabotropic glutamate receptors), and α‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazolepropionic acid (an agonist of ionotropic glutamate receptors) stimulated also tyrosine phosphorylation of pp110 and pp120. These effects were not observed in astrocytes in primary culture. In hippocampal slices tyrosine phosphorylation of pp110 and pp120 was stimulated by Ca 2+ ‐ionophores and by phorbol esters and antagonized by a chelator of intracellular Ca 2+ and by drugs that inhibit protein kinase C. Stimulation of muscarinic and α 1 ,‐adrenergic receptors increased also tyrosine phosphorylation of pp110 and pp120. These results demonstrate that membrane depolarization and stimulation of neurotransmitter receptors activate a tyrosine phosphorylation pathway in neurons. This pathway involves an increase in intracellular Ca 2+ concentrations and the activation of protein kinase C. It may provide a biochemical basis for some neurotrophic effects of electrical activity and neurotransmitters and may contribute to the role of tyrosine phosphorylation in long‐term potentiation.