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Distribution of fetal erythroblasts in maternal blood after chorionic villous sampling
Author(s) -
AlMufti Raghad,
Hambley Henry,
Farzaneh Farzin,
Nicolaides Kypros H.
Publication year - 2003
Publication title -
bjog: an international journal of obstetrics and gynaecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.157
H-Index - 164
eISSN - 1471-0528
pISSN - 1470-0328
DOI - 10.1046/j.1471-0528.2003.02204.x
Subject(s) - fetus , chorionic villus sampling , andrology , giemsa stain , fluorescence in situ hybridization , nucleated red blood cell , gestation , biology , prenatal diagnosis , medicine , microbiology and biotechnology , pregnancy , pathology , chromosome , genetics , gene
Objective To investigate whether chorionic villus sampling (CVS) is associated with an increase in fetomaternal cell trafficking. Design Prospective study. Setting King's College London School of Medicine, King's College Hospital. Sample Eighteen singleton pregnancies undergoing CVS for fetal karyotyping at 11–14 weeks of gestation and subsequently found to have chromosomal defects. Method Maternal blood samples were obtained immediately before and at 3–14 (median 5) days after CVS. Fetal erythroblasts were isolated using triple density gradient separation and anti‐CD71 magnetic cell sorting techniques. The enriched erythroblasts were stained with Kleihauer–Giemsa and with fluorescent antibodies for the epsilon (ɛ) and gamma (γ) globin chains. The percentage of fetal cells positive for each stain was calculated. Fluorescence in situ hybridisation (FISH) for X‐ and Y‐chromosomes was also performed. Comparison was made in the proportion of enriched fetal cells between the pre‐CVS and post‐CVS samples. Main outcome measures The proportion of fetal erythroblasts in maternal blood. Results The percentage of erythroblasts enriched from maternal blood that stained positive for ɛ and γ globin chains and with Kleihauer–Giemsa was significantly higher in the post‐CVS samples compared with the pre‐CVS samples. FISH analysis for the Y‐chromosome confirmed the increase in fetal cell proportion in the post‐CVS samples. The percentage difference in fetal cells decreased significantly with time interval from CVS. Conclusion CVS results in an increase in fetomaternal cell trafficking, which continues to be present for several days after the procedure.