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Ni‐induced oxidative stress in roots of the Ni hyperaccumulator, Alyssum bertolonii
Author(s) -
Boominathan Rengasamy,
Doran Pauline M.
Publication year - 2002
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1046/j.1469-8137.2002.00506.x
Subject(s) - catalase , nicotiana tabacum , superoxide dismutase , hyperaccumulator , lipid peroxidation , oxidative stress , reactive oxygen species , biology , endogeny , botany , chemistry , biochemistry , gene , ecology , contamination , soil contamination
Summary• The aim of this study was to determine whether superior antioxidative defences contribute to Ni tolerance in roots of the hyperaccumulator species, Alyssum bertolonii. Antioxidative responses were compared in hairy roots ofA. bertoloniiand the nonhyperaccumulator,Nicotiana tabacum . • Growth, Ni uptake, antioxidative enzyme activities, lipid peroxidation and concentrations of H 2 O 2 and surface ‐SH groups were measured in hairy root cultures exposed to 25 ppm (426 µ m ) Ni. • Growth of A. bertoloniiroots was not affected by Ni, whereas Ni preventedN. tabacumroot growth. Endogenous activities of superoxide dismutase and catalase were 2.4 and > 500 times greater, respectively, inA. bertoloniiroots than inN. tabacum. H2 O 2 levels rose significantly with Ni treatment in both species, by factors of 3.6 and 8.6, respectively. • Compared with N. tabacum, oxidative damage may be minimised inA. bertoloniiroots by high endogenous activities of catalase and, to a lesser extent, superoxide dismutase. As accumulation of H2 O 2 was not detrimental to A. bertolonii , enhanced mechanisms for tolerating active oxygen species may also be present.