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Trehalose induces resistance to powdery mildew in wheat
Author(s) -
Reignault PH.,
Cogan A.,
Muchembled J.,
LounesHadj Sahraoui A.,
Durand R.,
Sancholle M.
Publication year - 2001
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1046/j.1469-8137.2001.00035.x
Subject(s) - trehalose , blumeria graminis , powdery mildew , cinnamyl alcohol dehydrogenase , appressorium , biology , phenylalanine ammonia lyase , botany , peroxidase , alcohol dehydrogenase , germination , microbiology and biotechnology , biochemistry , enzyme , plant disease resistance , biosynthesis , gene
Summary• Reduction in the degree of powdery mildew infection of wheat leaves is observed after treatments with trehalose, a nonreducing disaccharide commonly found in a wide variety of organisms, including fungi. • Wheat ( Triticum aestivum ) cv. Sideral plants grown in phytotrons were inoculated with Blumeria graminis f.sp. tritici . In addition to degree of infection, the effect of trehalose solution was further investigated using light and fluorescence microscopy and enzyme assays. • Infection in wheat leaves was reduced by 50 and 95% with trehalose solution (15 g l −1 ) following a single spraying and three sprayings, respectively; in a detached leaf assay, trehalose was effective at concentrations as low as 0.01 g l −1 . Trehalose did not inhibit conidial germination and differentiation of appressoria ( in vitro or on the leaf epidermis), but enhanced papilla deposition in epidermal cells. Trehalose also enhanced phenylalanine ammonia‐lyase (PAL) and peroxidase (PO) activities; both markers of plant defence responses. However, the level of three cinnamyl alcohol dehydrogenase (CAD) activities (conyferyl, p ‐coumaryl and sinapyl alcohol dehydrogenase) was unchanged. • Trehalose treatment of wheat confers resistance to B. graminis infection by activating plant defence responses (e.g. papilla deposition, PAL and PO activities).