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Expression of chitin synthase genes in the arbuscular mycorrhizal fungus Gigaspora margarita
Author(s) -
LANFRANCO L.,
VALLINO M.,
BONFANTE P.
Publication year - 1999
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1046/j.1469-8137.1999.00393.x
Subject(s) - chitin , chitin synthase , biology , gene , spore germination , fungus , genomic dna , microbiology and biotechnology , transcription (linguistics) , biochemistry , spore , botany , linguistics , philosophy , chitosan
Genomic DNA from spores of the endomycorrhizal fungus Gigaspora margarita was amplified with two sets of degenerate primers designed on conserved regions of chitin synthase polypeptides. Three chitin synthase ( chs ) gene portions were identified: Gimchs 1, Gimchs 2 and Gimchs 3, encoding one class II and two class IV chitin synthases, respectively. Gimchs 1 and Gimchs 3 transcripts were detected by reverse transcription polymerase chain reaction (RT‐PCR) during root colonization, whereas none of three mRNAs was revealed during spore germination. The potential role of the genes expressed is discussed in the light of morphogenetic and signalling events.