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Looking for molecular and physiological markers of osmotic adjustment in sunflower
Author(s) -
JAMAUX I.,
STEINMETZ A.,
BELHASSEN E.
Publication year - 1997
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1046/j.1469-8137.1997.00817.x
Subject(s) - biology , rapd , molecular marker , sunflower , desiccation , genetic marker , botany , genetics , horticulture , gene , genetic diversity , population , demography , sociology
summary Two families of Helianthus lines, T −S (low desiccation rate) and T + (low desiccation rate), with contrasting osmotic adjustment (OA), obtained from a divergent selection programme, were compared for several morphological traits, ecophysiological parameters and molecular markers. The physiological analysis in a glasshouse experiment showed that the two families were identical in irrigated conditions except for a single trait, relative water loss (RWL: water loss rate of excised leaves). RWL could be used for these genotypes as a physiological marker of OA. A molecular analysis of the two contrasting lines, using differential screening of cDNA libraries, led to the isolation of three specific cDNAs, one of which, DRS26, showed high sequence homology with a mammalian amino‐acid transporter. Subsequent RFLP and STS studies, using DRS26 and RAPD bulked analysis, enabled the identification of putative molecular markers of RWL and OA in T − :T + sunflower.