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Immunohistochemical localization of carbonic anhydrase isozymes in the rat carotid body
Author(s) -
Yamamoto Yoshio,
Fujimura Minako,
Nishita Toshiho,
Nishijima Kazutoshi,
Atoji Yasuro,
Suzuki Yoshitaka
Publication year - 2003
Publication title -
journal of anatomy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 118
eISSN - 1469-7580
pISSN - 0021-8782
DOI - 10.1046/j.1469-7580.2003.00191.x
Subject(s) - carotid body , carbonic anhydrase , isozyme , immunohistochemistry , glomus cell , vascular smooth muscle , chemistry , pathology , carbonic anhydrase ii , anatomy , biology , smooth muscle , endocrinology , enzyme , medicine , biochemistry , stimulation
The rat carotid body was immunohistochemically stained for carbonic anhydrase I, II and III (CA‐I, CA‐II and CA‐III). Immunoreactivity for CA‐I was distributed in type I cells, type II cells and nerve bundles. Smooth muscle cells and endothelial cells of blood vessels were also strongly stained for CA‐I. CA‐II immunoreactivity was distinctly positive in type I cells and nerve bundles. Vascular smooth muscle cells were weakly positive, and type II cells were negative for CA‐II. CA‐III immunoreactivity was identified in type I cells and vascular smooth muscle cells. Our results suggest that carbonic anhydrase isozymes in type I cells play an important role in chemoreception for hypercapnia. Immunoreactivities for CA‐I and CA‐II in the nerve fibres may participate in the synergic action of carotid sinus nerve between hypoxic and hypercapnic stimuli.