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Cardiomyocyte differentiation of mouse and human embryonic stem cells *
Author(s) -
Mummery C.,
Ward D.,
Van Den Brink C. E.,
Bird S. D.,
Doevendans P. A.,
Opthof T.,
De La Riviere A. Brutel,
Tertoolen L.,
Van Der Heyden M.,
Pera M.
Publication year - 2002
Publication title -
journal of anatomy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 118
eISSN - 1469-7580
pISSN - 0021-8782
DOI - 10.1046/j.1469-7580.2002.00031.x
Subject(s) - embryonic stem cell , stem cell , induced pluripotent stem cell , cardiac function curve , p19 cell , transplantation , microbiology and biotechnology , biology , cellular differentiation , ischemia , medicine , cancer research , pathology , immunology , heart failure , genetics , gene
Ischaemic heart disease is the leading cause of morbidity and mortality in the western world. Cardiac ischaemia caused by oxygen deprivation and subsequent oxygen reperfusion initiates irreversible cell damage, eventually leading to widespread cell death and loss of function. Strategies to regenerate damaged cardiac tissue by cardiomyocyte transplantation may prevent or limit post‐infarction cardiac failure. We are searching for methods for inducing pluripotent stem cells to differentiate into transplantable cardiomyocytes. We have already shown that an endoderm‐like cell line induced the differentiation of embryonal carcinoma cells into immature cardiomyoctyes. Preliminary results show that human and mouse embryonic stem cells respond in a similar manner. This study presents initial characterization of these cardiomyocytes and the mouse myocardial infarction model in which we will test their ability to restore cardiac function.