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Early nephron formation in the developing mouse kidney
Author(s) -
BARD JONATHAN B. L.,
GORDON ADELE,
SHARP LINDA,
SELLERS WILLIAM I.
Publication year - 2001
Publication title -
journal of anatomy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 118
eISSN - 1469-7580
pISSN - 0021-8782
DOI - 10.1046/j.1469-7580.2001.19940385.x
Subject(s) - nephron , mesenchyme , kidney , duct (anatomy) , kidney development , microbiology and biotechnology , confocal microscopy , epithelium , pathology , biology , anatomy , chemistry , medicine , mesenchymal stem cell , endocrinology , biochemistry , embryonic stem cell , gene
This paper reports 3‐dimensional confocal microscopy observations on how nephrogenic aggregates form from the NCAM‐ and Pax2‐positive caps (4–5 cells deep) of condensed metanephric mesenchyme surrounding the duct tips of the mouse kidney. Aggregates of 6–8 cells are first seen at ∼E12.5–12.75 immediately proximal to this cap, closely abutting the duct surface. As the tip advances, NCAM expression is maintained in the cap but is otherwise restricted to aggregates whose cells rapidly epithelialise, forming tubules that invade the duct epithelium. Pax2 expression studies shows how the rind of nephrogenic blastemal cells forms: as duct tips extend towards the kidney surface, the associated Pax2+ cells form patches of cells on the kidney surface. These observations revise our knowledge of the timing and process of nephron initiation.