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Appearance of biomarkers of in vitro ageing after successive stimulation of WI‐38 fibroblasts with IL‐1α and TNF‐α: senescence associated β‐galactosidase activity and morphotype transition
Author(s) -
DUMONT PATRICK,
BALBEUR LAURA,
REMACLE JOSE,
TOUSSAINT OLIVIER
Publication year - 2000
Publication title -
journal of anatomy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 118
eISSN - 1469-7580
pISSN - 0021-8782
DOI - 10.1046/j.1469-7580.2000.19740529.x
Subject(s) - senescence , stimulation , ageing , fibroblast , intracellular , reactive oxygen species , oxidative stress , in vitro , tumor necrosis factor alpha , microbiology and biotechnology , biology , immunology , biochemistry , endocrinology , genetics
Sublethal oxidative stresses increase the proportions of human fibroblasts positive for senescence associated β‐galactosidase activity and accelerate the transition in the fibroblast morphotypes characterising fibroblast ageing. Stimulation of fibroblasts with TNF‐α or IL‐1α transiently increases the production of reactive oxygen species (ROS) in human fibroblasts. Here we propose that repeated stimulation of WI‐38 fibroblasts with TNF‐α or IL‐1α can generate enough ROS to accelerate the transition in the fibroblast morphotypes and increase the proportion of cells positive for senescence associated β‐galactosidase activity. The involvement of ROS is suggested by experiments where the stimulation of fibroblasts with TNF‐α or IL‐1α are performed in the presence of N‐acetylcysteine which increases the intracellular antioxidant potential. It is proposed that the decrease in the proportions of morphotypes I and II, and the increase in the proportions of morphotypes III to VI observed after successive stimulation with TNF‐α or IL1‐α is attributed to an increased ROS production occurring during the stimulation.