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Parvalbumin in cortical epithelial cells of the pigeon thymus
Author(s) -
ATOJI YASURO,
YAMAMOTO YOSHIO,
SUZUKI YOSHITAKA
Publication year - 2000
Publication title -
journal of anatomy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 118
eISSN - 1469-7580
pISSN - 0021-8782
DOI - 10.1046/j.1469-7580.2000.19630305.x
Subject(s) - parvalbumin , cytoplasm , nucleus , euchromatin , ultrastructure , organelle , biology , cell type , vacuole , electron microscope , microbiology and biotechnology , anatomy , cell , heterochromatin , chromatin , neuroscience , dna , genetics , physics , optics
We examined the distribution of parvalbumin in the pigeon thymus by light and electron microscopic immunohistochemistry. Tissues were also examined by conventional electron microscopy to determine the ultrastructure of immunoreactive cells. Parvalbumin immunoreaction was located in epithelial cells of the cortex, which formed dense mesh‐like structures. Parvalbumin‐positive epithelial cells were classified into 2 types. The first comprised elongated cells. In these, the nucleus was spindle‐shaped, oval, or triangular, with a slightly irregular contour and contained rich heterochromatin peripherally. The cytoplasm was pale and processes extended laterally or ramified among the surrounding thymocytes. This type of cell formed the majority of immunoreactive cells. The other cell type consisted of polygonal epithelial cells. The nucleus was oval with deep indentations. Euchromatin occupied a large part of the nucleus. The cytoplasm contained numerous cell organelles compared with the elongated type, in particular, electron‐dense vacuoles of various sizes and often bundles of tonofilaments. Both types of epithelial cell were interconnected by desmosomes. No secretory granules were found in the cytoplasm of elongated or polygonal cells. These results indicate the presence of heterogeneous group of parvalbumin‐immunoreactive epithelial cells and suggest the likelihood of different functional roles for parvalbumin in the pigeon thymus.