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Temporal expression of the matrix metalloproteinase MMP‐2 correlates with fibronectin immunoreactivity during the development of the vascular system in the chick embryo chorioallantoic membrane
Author(s) -
RIBATTI DOMENICO,
NICO BEATRICE,
VACCA ANGELO,
IURLARO MONICA,
RONCALI LUISA
Publication year - 1999
Publication title -
journal of anatomy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 118
eISSN - 1469-7580
pISSN - 0021-8782
DOI - 10.1046/j.1469-7580.1999.19510039.x
Subject(s) - fibronectin , chorioallantoic membrane , angiogenesis , laminin , matrix metalloproteinase , extracellular matrix , embryo , biology , microbiology and biotechnology , matrix (chemical analysis) , metalloproteinase , type iv collagen , in vivo , embryogenesis , immunohistochemistry , pathology , chemistry , immunology , biochemistry , medicine , genetics , chromatography
In this study we have examined in the chick embryo chorioallantoic membrane (ChAM) the expression of the matrix metalloproteinase 2 (MMP‐2) and have correlated this parameter with the expansion of the ChAM vasculature and with the expression of 3 extracellular matrix components (fibronectin, laminin and type IV collagen), which differentially modulate angiogenesis. In the early phases of ChAM development, between d 6 and d 8 of incubation, when the increase of the ChAM vasculature is maximal, higher values of MMP‐2 and, respectively, of fibronectin immunoreactive area, are detectable. These results indicate that MMP‐2 activity and fibronectin expression are 2 strictly related components of angiogenesis occurring in vivo.