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Lectin binding patterns in nonsensory regions of rat cochlea during postnatal development
Author(s) -
KHAN KHALID M.,
SARFARAZ NOORJEHAN,
SALIM ZEENAT
Publication year - 1999
Publication title -
journal of anatomy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 118
eISSN - 1469-7580
pISSN - 0021-8782
DOI - 10.1046/j.1469-7580.1999.19440497.x
Subject(s) - staining , cochlea , lectin , immunostaining , biology , spiral ligament , glycoconjugate , microbiology and biotechnology , anatomy , biochemistry , immunohistochemistry , immunology , organ of corti , genetics
The distribution of glycoconjugates was examined in the nonsensory regions of the rat cochlea during postnatal development using biotin‐conjugated lectins. Temporal bones of rats at postnatal d 1 and at wk 2, 4 and 6 were fixed in 4% paraformaldehyde and 0.1% glutaraldehyde and processed for paraffin wax embedding. The dewaxed sections were incubated with 7 biotinylated lectins, followed by avidin‐biotin‐peroxidase complex. A different staining pattern was observed in the stria vascularis, spiral ligament and spiral limbus in the age groups examined. The staining intensity varied between lectins and the reaction product exhibited limited disparity. The staining intensity for WGA increased with age in all the 3 nonsensory regions. The staining patterns for the other lectins differed in the various nonsensory regions examined indicating tissue specificity. The limited variations in the lectin binding patterns after 2nd wk of postnatal life also indicate that the changes in the carbohydrate moieties are established during the fetal period of cochlear development and limited changes take place during postnatal maturation of the nonsensory regions.