Premium
Quantitative analysis of the sympathetic innervation of the rat knee joint
Author(s) -
CATRE MEL G.,
SALO PAUL T.
Publication year - 1999
Publication title -
journal of anatomy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 118
eISSN - 1469-7580
pISSN - 0021-8782
DOI - 10.1046/j.1469-7580.1999.19420233.x
Subject(s) - retrograde tracing , efferent , tyrosine hydroxylase , vasoactive intestinal peptide , population , somatostatin , sympathetic nervous system , anatomy , endocrinology , medicine , immunohistochemistry , biology , neuropeptide , receptor , afferent , blood pressure , environmental health
Retrograde tracing with Fluoro‐Gold (FG) was used to identify the complete population of knee joint sympathetic postganglionic efferents in the lumbar sympathetic chain of adult female Wistar rats. In 6 rats, the total number and distribution of FG‐labelled neurons in the lumbar sympathetic chain was determined. The rat knee joint is supplied by an average of 187±57 sympathetic afferents with the majority at the L3 and L4 levels. Immunohistochemistry using antibodies specific for tyrosine hydroxylase (TH), somatostatin (SS) or vasoactive intestinal polypeptide (VIP) revealed that 33% of knee joint sympathetic afferents contained TH, 42% contained VIP, and none contained somatostatin. Retrograde tracing with FG provided accurate and reproducible labelling of the joint‐innervating subpopulation of sympathetic efferent neurons. This model lends itself to the further study of the molecular responses of this neuronal population in the various disorders and conditions affecting joints.