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In vivo fibre optic confocal imaging of microvasculature and nerves in the rat vas deferens and colon
Author(s) -
PAPWORTH G. D.,
DELANEY P. M.,
BUSSAU L. J.,
VO L. T.,
KING R. G.
Publication year - 1998
Publication title -
journal of anatomy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 118
eISSN - 1469-7580
pISSN - 0021-8782
DOI - 10.1046/j.1469-7580.1998.19240489.x
Subject(s) - vas deferens , confocal , confocal microscopy , pathology , anatomy , in vivo , optic nerve , magnification , medicine , biology , computer science , microbiology and biotechnology , geometry , mathematics , computer vision
A fluorescence confocal microscopy technique was employed to obtain subsurface images of nerve and microvascular structure in the vas deferens and colon of the living rat. The use of dual labelling with vital dyes and 2‐channel confocal acquisition allowed differentiation of microscopic structure at both low and higher magnification. Characteristic staining patterns of nerves and blood vessels were repeatedly obtained in each tissue, suggesting the potential of this technique for studying morphological changes associated with surgical procedures and/or models of neuronal or vascular pathology.