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Detection and quantification of HIV‐1 specific CD4 helper and CD8 cytotoxic cells: their role in HIV‐1‐infected individuals and vaccine recipients
Author(s) -
Imami N,
Hardy G,
Pires A,
Burton C,
Sullivan A,
Gotch F
Publication year - 2001
Publication title -
hiv medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.53
H-Index - 79
eISSN - 1468-1293
pISSN - 1464-2662
DOI - 10.1046/j.1468-1293.2001.00068.x
Subject(s) - cytotoxic t cell , cd8 , medicine , immunology , immunotherapy , vaccination , human immunodeficiency virus (hiv) , virology , hiv vaccine , cd4 cd8 ratio , t cell , immune system , in vitro , biology , vaccine trial , lymphocyte subsets , biochemistry
There is a strong link between virus specific CD8 T‐cell function and the efficiency of regulatory CD4 helper T cells. Controlling viraemia in HIV‐1‐infected individuals requires the maintenance of strong CD4 and CD8 T‐cell responses. These responses should be elicited by prophylactic vaccination and by postexposure immunotherapy. This review will examine the methods that are available for the detection and quantification of HIV‐1 specific CD4 and CD8 T‐cell responses. We will also discuss the methods that should be used to identify these responses in HIV‐1‐infected individuals, seropositive recipients of immunotherapy and seronegative vaccinees. Finally, we will give examples of how responses observed in vitro relate to those known to occur in vivo .