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Octapeptide but not nonapeptide from HIV‐1 p24 gag protein upregulates cell surface HLA‐C expression
Author(s) -
Kozłowska A,
Gorczyca W,
Maćkiewicz Z,
Wojciechowska I,
Kuśnierczyk P
Publication year - 2000
Publication title -
hiv medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.53
H-Index - 79
eISSN - 1468-1293
pISSN - 1464-2662
DOI - 10.1046/j.1468-1293.2000.00029.x
Subject(s) - human leukocyte antigen , downregulation and upregulation , microbiology and biotechnology , clone (java method) , antibody , cell , cell culture , medicine , antigen , biology , immunology , biochemistry , gene , genetics
Objectives The HLA‐Cw3 molecule has been reported to present peptides derived from HIV‐1 p24 gag protein to a cytotoxic T lymphocyte clone. We have shown previously that the synthetic octapeptide 145–152 derived from the p24 gag sequence upregulated cell surface HLA‐C expression on HLA‐Cw*0303 + cells. Here, we examined the question of whether the nonapeptide 144–152 also exerts a similar effect. Methods The HLA‐Cw*0303 + B‐LCL PAJ and control HLA‐Cw3‐negative cells B‐LCL HAJ and T‐LCL 500/C9 were used. HLA expression on peptide‐pulsed and non‐pulsed cells was evaluated using specific antibodies and flow cytofluorimetry. Binding of dansylated peptides onto different cell lines was measured spectrofluorimetrically. Results The HIV‐1 p24 gag octapeptide upregulated cell surface HLA‐C on PAJ (Cw*0303 + ) cells, whereas the nonapeptide did not. HLA‐A2 expression was not affected by these peptides. Specificity of the effect of octapeptide was confirmed by the lack of HLA‐C upregulation on HLA‐Cw3 – cells and by lower binding of dansylated petide to the HLA‐Cw3 ‐ cells HAJ and 500/C9. Conclusions The above results indicate that HLA‐Cw*0303 preferentially binds the octapeptide rather than the nonapeptide derived from HIV‐1 p24 gag protein .