Open AccessA sequence mutation in the cinnamyl alcohol dehydrogenase gene associated with altered lignification in loblolly pine
Author(s) -
Gill Geoffrey P.,
Brown Garth R.,
Neale David B.
Publication year - 2003
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1046/j.1467-7652.2003.00024.x
Subject(s) - cinnamyl alcohol dehydrogenase , biology , exon , loblolly pine , cinnamyl alcohol , genetics , gene , alcohol dehydrogenase , microbiology and biotechnology , botany , biochemistry , alcohol , biosynthesis , pinus <genus> , catalysis , selectivity
Summary Evidence for the molecular basis of a null allele of cinnamyl alcohol dehydrogenase (CAD) has been discovered in the loblolly pine ( Pinus taeda L.) clone 7‐56. The mutation is a two‐base pair adenosine insertion located in exon 5 that causes a frame‐shift which is predicted to result in premature termination of the protein. For routine detection of the mutation, a diagnostic assay was developed utilizing T emplate‐directed D ye‐terminator I ncorporation and F luorescence P olarization detection (FP‐TDI). Loblolly pine is the most important commercial tree species in the USA, being harvested for pulp and solid wood products. Chemical pulping could be increased in efficiency by selecting for trees having a two‐base pair adenosine insertion, by use of the rapid diagnostic assay developed in this study.