Open AccessSite‐independently integrated transgenes in the elite restorer rice line Minghui 63 allow removal of a selectable marker from the gene of interest by self‐segregation
Author(s) -
Tu Jumin,
Datta Karabi,
Oliva Norman,
Zhang Guoan,
Xu Caiguo,
Khush Gurdev Singh,
Zhang Qifa,
Datta Swapan Kumar
Publication year - 2003
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1046/j.1467-7652.2003.00012.x
Subject(s) - selectable marker , biology , locus (genetics) , gene , transgene , genetics , marker gene , southern blot
Summary In this study, we have demonstrated that two independent loci are involved in the integration of the insecticidal protein gene cryIAb/cryIAc and selectable marker gene hph in the recipient genome of the elite Chinese CMS restorer line Minghui 63. We have also documented the structural organization of these transgenes in each locus by restriction enzyme digestion and Southern blot analysis. The independent locus integration of different transgenes allowed us to remove the selectable marker gene hph from the gene of interest simply by self‐segregation. Not having the selectable marker gene will enhance the commercial value of our transgenic line TT51‐1, which showed a consistently high level of resistance against repeated infestations of yellow stem borers and natural outbreaks of leaf‐folders, without a reduction in yield potential.