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Simultaneous determination of four anti‐dandruff agents including octopirox in shampoo products by reversed‐phase liquid chromatography
Author(s) -
Chao L.
Publication year - 2001
Publication title -
international journal of cosmetic science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.532
H-Index - 62
eISSN - 1468-2494
pISSN - 0142-5463
DOI - 10.1046/j.1467-2494.2001.00090.x
Subject(s) - chromatography , dandruff , shampoo , chemistry , high performance liquid chromatography , potassium phosphate , acetonitrile , potassium , reversed phase chromatography , organic chemistry
Synopsis A method based on reversed‐phase liquid chromatography (HPLC) has been developed for the simultaneous identification and quantitative determination of four anti‐dandruff agents such as salicylic acid, ketoconazole, climbazole, octopirox in commercial anti‐dandruff shampoo products. A symmetry C18 column (5 µm, 250 mm × 4.6 mm i.d.) was used at temperature of 35 °C, mobile phase with flow rate of 0.8 mL min −1 was acetonitrile: water (containing 10 m m potassium dihydrogen phosphate, pH 4.0, adjusted with orthophosphoric acid) = 60 : 40 (V/V) and UV detection at 224 nm and 305 nm. Samples were extracted with mobile phase by stirring and ultrasonic method. The average recoveries of four anti‐dandruff agents were 98.0–104.1%. The relative standard deviations for samples were 0.11–0.90%. The method is simple, rapid and reproducible.