Open AccessCore promoter for initiation of Cucumber mosaic virus subgenomic RNA4A
Author(s) -
Sivakumaran K.,
Chen M.H.,
Roossinck M. J.,
Kao C. C.
Publication year - 2002
Publication title -
molecular plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.945
H-Index - 103
eISSN - 1364-3703
pISSN - 1464-6722
DOI - 10.1046/j.1464-6722.2001.00089.x
Subject(s) - subgenomic mrna , biology , sequence (biology) , rna , virology , rna dependent rna polymerase , cucumber mosaic virus , microbiology and biotechnology , genetics , computational biology , virus , plant virus , gene
summary The core subgenomic promoter for the initiation of Cucumber mosaic virus (CMV) RNA4A was characterized in vitro using a template‐dependent RNA synthesis assay and variants of the core promoter RNAs. The minimal sequence required for specific initiation from the cytidylate (T1) used in vivo consists of 31‐nucleotides (nt) 3′ of T1 and a 13 nt template sequence. This 44 nt RNA was found to provide three elements that contribute to efficient initiation of RNA4A synthesis by the CMV replicase: a stem‐loop secondary structure 3′ of T1, a template sequence that is rich in adenylates and uridylates, and T1 in an unbase‐paired sequence.