Open AccessThe barley powdery mildew protein kinase C gene, pkc 1 and pkc ‐like gene, are differentially expressed during morphogenesis
Author(s) -
Zhang Ziguo,
Priddey Gemma,
Gurr Sarah Jane
Publication year - 2001
Publication title -
molecular plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.945
H-Index - 103
eISSN - 1364-3703
pISSN - 1464-6722
DOI - 10.1046/j.1464-6722.2001.00081.x
Subject(s) - protein kinase c , powdery mildew , biology , blumeria graminis , gene , mildew , complementary dna , phorbol , microbiology and biotechnology , protein kinase a , kinase , genetics , botany , plant disease resistance
summary Protein kinase C agonist assays revealed the phorbol ester, phorbol 12‐myristate 13‐acetate, invoked germling morphogenesis and enhanced PKC activity in Blumeria graminis . No antagonist of mildew PKC activity was found but the data fuelled a hunt for powdery mildew pkc genes. Oligonucleotides, designed on the basis of conserved ATP‐binding and kinase domains within the catalytic core of eukaryotic protein kinase proteins, were used as primers to amplify chromosomal and cDNA fragments from the barley powdery mildew fungus graminis . Three kinase gene fragments were isolated ( pkc 1, pkc ‐like and c pka ) and the full length genomic sequences of the mildew pkc and pkc‐ like genes were determined by ‘step down’ PCR. RT‐PCR transcript profiles showed the three genes to be differentially regulated during germling morphogenesis.