Premium
Associations of LDL size with in vitro oxidizability and plasma levels of in vivo oxidized LDL in Type 2 diabetic patients
Author(s) -
Scheffer P. G.,
Bos G.,
Volwater H. G. F. M.,
Dekker J. M.,
Heine R. J.,
Teerlink T.
Publication year - 2003
Publication title -
diabetic medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.474
H-Index - 145
eISSN - 1464-5491
pISSN - 0742-3071
DOI - 10.1046/j.1464-5491.2003.00975.x
Subject(s) - in vivo , medicine , low density lipoprotein , endocrinology , in vitro , apolipoprotein b , cholesterol , lipoprotein , oxidative phosphorylation , ldl cholesterol , chemistry , biochemistry , biology , microbiology and biotechnology
Aims Oxidative modification of low‐density lipoprotein (LDL) is believed to be a key step in the genesis of atherosclerotic lesions. The presence of small, dense LDL is associated with accelerated atherosclerosis and is common in diabetic patients. The aim of this study was to investigate the relationship of in vitro LDL oxidizability and circulating in vivo oxidized LDL with LDL particle size in Type 2 diabetic patients and healthy control subjects. Subjects and methods The study group consisted of 58 elderly well controlled Type 2 diabetic patients and 58 control subjects with normal glucose metabolism. LDL particle size was measured by high‐performance gel‐filtration chromatography. In vitro oxidizability of LDL was measured by monitoring conjugated diene formation and plasma levels of circulating oxidized LDL were determined by ELISA. Results In vitro susceptibility of LDL to oxidation was not related to plasma levels of in vivo oxidized LDL, nor to LDL particle size. In the diabetic patients, but not in the control group, an inverse relation between LDL size and in vivo oxidized LDL was observed ( r = −0.35, P = 0.007). This relation was strengthened after controlling for LDL‐cholesterol concentration ( r = −0.52, P < 0.001). Conclusions In agreement with the view that small, dense LDL accelerates atherosclerosis, an inverse relationship was observed between LDL size and circulating in vivo oxidized LDL in Type 2 diabetic patients. Our results also suggest that in vitro susceptibility to oxidation is not a suitable surrogate measure for in vivo LDL oxidation. Diabet. Med. 20, 563–567 (2003)