Premium
Increased secretion of TGF‐β1 by peripheral blood mononuclear cells from patients with Type 1 diabetes mellitus with diabetic nephropathy
Author(s) -
Korpinen E.,
Groop P. H.,
Fagerudd J. A.,
Teppo A. M.,
Åkerblom H. K.,
Vaarala O.
Publication year - 2001
Publication title -
diabetic medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.474
H-Index - 145
eISSN - 1464-5491
pISSN - 0742-3071
DOI - 10.1046/j.1464-5491.2001.00413.x
Subject(s) - medicine , microalbuminuria , diabetic nephropathy , endocrinology , nephropathy , peripheral blood mononuclear cell , excretion , diabetes mellitus , biology , in vitro , biochemistry
SummaryAims To study transforming growth factor (TGF)‐β1 secretion by peripheral blood mononuclear cells (PBMC) from Type 1 diabetic patients with and without nephropathy. Methods Thirty normoalbuminuric Type 1 diabetic patients (urinary albumin excretion rate (AER) < 20 μg/min), 12 microalbuminuric (AER 20–200 μg/min), 10 nephropathic (AER > 200 μg/min), and 13 non‐diabetic individuals were recruited. TGF‐β1 secretion by PBMC was measured by enzyme immunoassay (EIA) after 48 h culture with and without phytohaemagglutinin (PHA) (5 μg/ml). Results After 48 h culture, the highest TGF‐β1 levels secreted by unstimulated PBMC were found in patients with nephropathy (median 6.2 (range 0.9–20.0) ng/ml) when compared to patients with normal albumin excretion (4.1 (0.2–11.3) ng/ml), microalbuminuria (1.8 (0.2–6.4) ng/ml) and healthy controls (1.0 (0.2–7.0) ng/ml); P = 0.02 for the three diabetic groups and P = 0.006 for all groups. At 48 h, the PHA‐stimulated TGF‐β1 levels were 12.4 (2.9–30.0) ng/ml in nephropathic, 7.3 (0.5–21.2) ng/ml in normoalbuminuric, and 5.5 (0.5–27.6) ng/ml in microalbuminuric patients ( P = 0.05). A correlation was observed between TGF‐β1 and diastolic blood pressure in the subgroup of patients with incipient and overt nephropathy ( r = 0.45, P = 0.04). Conclusions Type 1 diabetic patients with overt nephropathy show increased TGF‐β1 secretion by PBMC. Diastolic blood pressure levels correlated with TGF‐β1 secretion in dyabetic patients with nephropathy. Increased TGF‐β1 secretion by PBMC may be associated with renal and vascular disease in Type 1 diabetes mellitus.