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Polyphenols in red wine inhibit the proliferation and induce apoptosis of LNCaP cells
Author(s) -
Romero I.,
Páez A.,
Ferruelo A.,
Luján M.,
Berenguer A.
Publication year - 2002
Publication title -
bju international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.773
H-Index - 148
eISSN - 1464-410X
pISSN - 1464-4096
DOI - 10.1046/j.1464-410x.2002.02790.x
Subject(s) - rutin , gallic acid , tannic acid , quercetin , morin , apoptosis , chemistry , biochemistry , polyphenol , flavonoid , antioxidant , medicine , organic chemistry , pathology
Objective To assess the effect of five polyphenol constituents of red wine (quercetin, morin, rutin, gallic acid and tannic acid) on the proliferation of LNCaP cells, and to quantify the extent of apoptosis with each polyphenol. Materials and methods LNCaP cells (500) were cultured in microtitre plates and treated with gallic acid, tannic acid, quercetin (1, 5 and 10 µmol/L), rutin and morin (25, 50 and 75 µmol/L). A colorimetric immunoassay was then used to determine the extent of proliferation at 24, 48, 72 and 96 h, and a cell‐death detection assay to assess apoptosis at 24, 48 and 72 h. Results Gallic and tannic acid (5 and 10 µmol/L), morin (50 and 75 µmol/L), quercetin (5 and 10 µmol/L) and rutin (50 and 75 µmol/L) all significantly inhibited ( P <0.05) cell proliferation compared with the control. Apoptotic indexes were significantly greater ( P <0.01) in the presence of gallic (5 and 10 µmol/L) and tannic acid (5 and 10 µmol/L), and rutin (75 µmol/L, P <0.05) than in the control. The apoptotic effect of morin (75 µmol/L), although significant ( P <0.01), only appeared at 72 h. Conversely, while significant ( P <0.05) quercetin (5 and 10 µmol/L) had a transient (first 48 h) apoptotic effect compared with the control. Conclusion Quercetin, rutin, morin, gallic acid and tannic acid inhibited the growth of LNCaP cells at different concentrations, and induced apoptosis. The results provide a strong rationale for studying the in vivo effects of these compounds.