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Ischaemia‐reperfusion injury in the rat kidney: the effect of preconditioning
Author(s) -
ISLAM C.F.,
MATHIE R.T.,
DINNEEN M.D.,
KIELY E.A.,
PETERS A.M.,
GRACE P.A.
Publication year - 1997
Publication title -
british journal of urology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.773
H-Index - 148
eISSN - 1464-410X
pISSN - 0007-1331
DOI - 10.1046/j.1464-410x.1997.00209.x
Subject(s) - kidney , medicine , ischemia , renal artery , ischemic preconditioning , occlusion , acute tubular necrosis , urology , anesthesia
Objectives To design and establish a model to examine whether brief periods of renal artery occlusion (ischaemic preconditioning, IP) confers protection from the effects of a subsequent period of ischaemia and reperfusion of the rat kidney. Materials and methods Ninety rats were randomized into six groups, i.e. sham‐operated controls; IP alone; a 20 or 40 min period of left renal ischaemia (RI) alone; and IP followed by a 20 or 40 min period of RI. Preconditioning involved the sequential clamping of the left renal artery for 4 min and its release for 11 min, a total of four times, a ‘critical interval’ of 30 min before the ischaemic insult. Left renal tissue integrity was determined by dimercapto‐succinic acid (DMSA) radionuclide imaging on a γ‐camera both immediately (day 0) and 2 and 9 days later. Acute tubular necrosis was also assessed histologically. Results RI for 20 min resulted in a significant decrease in left renal tissue integrity on day 2 only ( P <0.001), whereas RI for 40 min caused significant left renal dysfunction on day 0, day 2 and day 9 ( P ≤0.01). For a given duration of ischaemia, there was no significant difference between results from (IP+RI) rats compared with RI‐only rats at any of the three times. There was no significant alteration in renal tissue integrity in the IP‐only rats compared with sham‐operated controls. Histological findings paralleled the data obtained from DMSA uptake. Conclusions The IP regimen and 30 min ‘critical interval’ confers no protection to the kidney from a 20 or 40 min ischaemic episode. The IP regimen itself appears to have no effect, confirming the validity of our experimental model.