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Use of nested polymerase chain reaction (PCR) for detection of retroviruses from formalin‐fixed, paraffin‐embedded uveal melanomas in cats
Author(s) -
Jean Stiles,
Dorothee Bienzle,
James A. Render,
NC Buyukmihci,
Emileigh Johnson
Publication year - 1999
Publication title -
veterinary ophthalmology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.594
H-Index - 50
eISSN - 1463-5224
pISSN - 1463-5216
DOI - 10.1046/j.1463-5224.1999.00066.x
Subject(s) - feline leukemia virus , feline immunodeficiency virus , polymerase chain reaction , cats , melanoma , dna , nested polymerase chain reaction , reverse transcriptase , virology , provirus , biology , microbiology and biotechnology , virus , sarcoma , pathology , viral disease , medicine , genome , lentivirus , cancer research , gene , genetics
Thirty‐six formalin‐fixed, paraffin‐embedded enucleated globes from cats with a diagnosis of diffuse anterior uveal melanoma were obtained. Sections of tumor were excised, deparaffinized, and subjected to nested polymerase chain reaction (PCR) to identify proviral DNA sequences from the feline leukemia virus (FeLV)–feline sarcoma virus (FeSV; 36 eyes), and the feline immunodeficiency virus (FIV; 18 eyes). All samples tested were negative for FIV DNA. Three samples were positive for FeLV–FeSV DNA. This is the first reported evidence of a possible link between naturally occurring feline anterior uveal melanoma and the presence of FeLV–FeSV DNA.