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Effects of rosiglitazone and oleic acid on UCP‐3 expression in L6 myotubes
Author(s) -
Costello A.,
Gray S.,
Donnelly R.
Publication year - 2003
Publication title -
diabetes, obesity and metabolism
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.445
H-Index - 128
eISSN - 1463-1326
pISSN - 1462-8902
DOI - 10.1046/j.1463-1326.2003.00255.x
Subject(s) - rosiglitazone , oleic acid , endocrinology , downregulation and upregulation , medicine , messenger rna , myogenesis , chemistry , myocyte , biochemistry , biology , receptor , gene
Aims: The regulation of uncoupling protein‐3 (UCP‐3) expression in muscle remains unclear, specifically in relation to dietary and drug treatments. The present study evaluated the effects of oleic acid and rosiglitazone on UCP‐3 mRNA expression in differentiated L6 myotubes. Methods: L6 myocytes were cultured and differentiated prior to exposure to rosiglitazone 10 µmol/l, oleic acid 100 µmol/l, or the combination, for 24 h, prior to semiquantitative evaluation of UCP‐3 mRNA relative to GAPDH mRNA by RT‐PCR. Results: Exposure to oleic acid produced a significant increase in UCP‐3 mRNA (0.012 ± 0.007 vs. 0.0011 ± 0.0006 for untreated cells, relative to GAPDH mRNA, p < 0.001). Rosiglitazone alone had no effect on UCP‐3 expression and nor did the glitazone affect oleic‐acid‐induced upregulation of UCP‐3. Conclusions: In L6 myotubes, 24‐h exposure to oleic acid produced a 10‐fold increase in UCP‐3 mRNA expression, but rosiglitazone had no effect. Oleic‐acid‐induced upregulation of UCP‐3 was not affected (positively or negatively) by glitazone exposure.