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β 3 ‐Adrenergic receptor gene polymorphism and type 2 diabetes in a Caucasian population
Author(s) -
Oeveren vanDybicz A. M,
Vonkeman H. E,
Bon M. A. M,
Van Den Bergh F. A. J. T. M,
Vermes I
Publication year - 2001
Publication title -
diabetes, obesity and metabolism
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.445
H-Index - 128
eISSN - 1463-1326
pISSN - 1462-8902
DOI - 10.1046/j.1463-1326.2001.00121.x
Subject(s) - medicine , endocrinology , type 2 diabetes , diabetes mellitus , genotype , beta 3 adrenergic receptor , population , obesity , body mass index , polymorphism (computer science) , type 2 diabetes mellitus , allele , allele frequency , biology , genetics , gene , adrenergic receptor , receptor , environmental health
SummaryAim The β 3 ‐adrenergic receptor (β 3 ‐AR) is suspected to play a key role in the regulation of energy balance by increasing lipolysis and thermogenesis. A mutation in the β 3 ‐AR gene (Trp64Arg) has been associated with the capacity of weight gain and with early onset of noninsulin dependent diabetes mellitus (type 2 diabetes). In this study we investigated the prevalence of the two β 3 ‐AR alleles in a Caucasian population and studied the association between the β 3 ‐AR genotype and metabolic disorders (obesity and type 2 diabetes). Methods Genomic DNA extracted from peripheral blood leucocytes of 200 Caucasian subjects (137 subjects with and 63 subjects without type 2 diabetes). The Mva I polymorphism of β 3 ‐AR, which detects the Trp64Arg mutation, was determined by polymerase chain reaction (PCR). We studied the correlation between the Trp64Arg mutation and the body mass index (b.m.i. kg/m 2 ). Results There was no significant difference between the patients with type 2 diabetes and control subjects in the frequency of the Arg64 allele (5.5% and 4.8%, respectively). Within the group of type 2 diabetes patients were 14 subjects with the Trp64Arg mutation (b.m.i., mean ± s.d.: 31 ± 8.5 kg/m 2 ) and 123 without the mutation (b.m.i. 29 ± 4.8). There was no association between the β 3 ‐AR gene polymorphism and sex, obesity, blood pressure, glycohaemoglobin concentration, proteinuria. Conclusion Our results suggest that the Trp64Arg mutation is not a major determinant of metabolic disorders (type 2 diabetes, obesity) and chronic complications of type 2 diabetes in a Dutch population.