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The variable effect on proliferation of a colon cancer cell line by the citrus fruit flavonoid Naringenin
Author(s) -
Frydoonfar H. R.,
McGrath D. R.,
Spigelman A. D.
Publication year - 2003
Publication title -
colorectal disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.029
H-Index - 89
eISSN - 1463-1318
pISSN - 1462-8910
DOI - 10.1046/j.1463-1318.2003.00444.x
Subject(s) - naringenin , flavonoid , cell growth , medicine , colorectal cancer , cell , cell culture , chemoprotective , flavanone , cancer , cancer research , biochemistry , biology , genetics , antioxidant
Objective Naringenin, a naturally occurring flavonoid found in citrus fruits, is known to have anticarcinogenic properties. We have examined the effect of Naringenin on cell proliferation of an HT‐29 colon cancer cell line. Methods HT‐29 colon cancer cells were cultured in 96‐well tissue culture plates. Naringenin concentrations ranging from 0.02 to 2.85 mmol were added to the wells of the Test group. The Control group contained all the elements present in the Test group with the exception of Naringenin. Cell proliferation was measured by colourimetric assay using the 2% WST‐1 cell proliferation kit. Results Significant inhibition of cell proliferation was observed in HT29 colon cancer cells exposed to Naringenin at doses greater than 0.71 mmol. Conclusions These results suggest a potential role for citrus fruits as a source of chemoprotective agents for colon cancer.