PKSP‐dependent reduction of phagolysosome fusion and intracellular kill of Aspergillus fumigatus conidia by human monocyte‐derived macrophages

Summary Previously, we described the isolation of an Aspergillus fumigatus mutant producing non‐pigmented conidia, as a result of a defective polyketide synthase gene, pksP ( polyketide synthase involved in pigment biosynthesis). The virulence of the pksP mutant was attenuated in a murine animal infection model and its conidia showed enhanced susceptibility towards damage by monocytes in vitro. Because macrophage‐mediated killing is critical for host resistance to aspergillosis, the interaction of both grey‐green wild‐type conidia and white pksP mutant conidia with human monocyte‐derived macrophages (MDM) was studied with respect to intracellular processing of ingested conidia. After phagocytosis, the percentage of wild‐type conidia residing in an acidic environment was approximately fivefold lower than that observed for non‐pigmented pksP mutant conidia. The phagolysosome formation, as assessed by co‐localization of LAMP‐1 and cathepsin D with ingested conidia, was significantly lower for wild‐type conidia compared with pksP mutant conidia. Furthermore, the intracellular kill of pksP mutant conidia was significantly higher than of wild‐type conidia, which was markedly increased by chloroquine, a known enhancer of phagolysosome fusion. Taken together, these findings suggest that the presence of a functional pksP gene in A. fumigatus conidia is associated with an inhibition of phagolysosome fusion in human MDM. These data show for the first time that a fungus has the capability to inhibit the fusion of the phagosome with the lysosome. This finding might help explain the attenuated virulence of pksP mutant strains in a murine animal model and provides a conceptual frame to understand the virulence of A. fumigatus .