Open AccessThe secreted effector protein of Salmonella dublin , SopA, is translocated into eukaryotic cells and influences the induction of enteritis
Author(s) -
Wood Michael W.,
Jones Michael A.,
Watson Patricia R.,
Siber Andrew M.,
McCormick Beth A.,
Hedges Samantha,
Rosqvist Roland,
Wallis Tim S.,
Galyov Edouard E.
Publication year - 2000
Publication title -
cellular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.542
H-Index - 138
eISSN - 1462-5822
pISSN - 1462-5814
DOI - 10.1046/j.1462-5822.2000.00054.x
Subject(s) - secretion , effector , biology , salmonella , microbiology and biotechnology , enteritis , virulence , type three secretion system , intestinal mucosa , secretory protein , phenotype , bacteria , gene , genetics , biochemistry , medicine
Salmonella ‐induced enteritis is associated with the induction of an acute intestinal inflammatory response and net fluid secretion into the lumen of infected mucosa. Proteins secreted by the Inv/Spa type III secretion system of Salmonella play a key role in the induction of these responses. We have demonstrated recently that the Inv/Spa‐secreted SopB and SopD effector proteins are translocated into eukaryotic cells via a Sipdependent pathway and act in concert to mediate inflammation and fluid secretion in infected ileal mucosa. Mutations of both sopB and sopD significantly reduced, but did not abrogate, the enteropathogenic phenotype. This indicated that other virulence factors are involved in the induction of enteritis. In this work, we characterize SopA, a secreted protein belonging to the family of Sop effectors of Salmonella dublin . We demonstrate that SopA is translocated into eukaryotic cells and provide evidence suggesting that SopA has a role in the induction of enteritis.