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Plant‐dependent active biological containment system for recombinant rhizobacteria
Author(s) -
Van Dillewijn Pieter,
Vílchez Susana,
Paz José A.,
Ramos Juan L.
Publication year - 2004
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1046/j.1462-2920.2003.00544.x
Subject(s) - rhizobacteria , biology , rhizosphere , pseudomonas putida , strain (injury) , pseudomonas , exudate , proline , population , microbiology and biotechnology , biological pest control , botany , bacteria , biochemistry , amino acid , genetics , anatomy , demography , sociology
Summary This work describes the construction of the rhizobacterium Pseudomonas putida strain CS‐4, which contains an active biological containment (ABC) system that ensures bacterial suicide in the absence of proline. Maize plants exudate proline, which allows CS‐4 to colonize the rhizosphere at a similar level to that of the wild‐type strain. However, when the plants are removed, the CS‐4 population decreased in bulk soil at a higher rate than the wild‐type strain.