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Diversity and distribution of a deeply branched novel proteobacterial group found in anaerobic–aerobic activated sludge processes
Author(s) -
Kong Yunhong,
Ong Say Leong,
Ng Wun Jun,
Liu WenTso
Publication year - 2002
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1046/j.1462-2920.2002.00357.x
Subject(s) - biology , enhanced biological phosphorus removal , activated sludge , proteobacteria , 16s ribosomal rna , anaerobic exercise , microbiology and biotechnology , phylogenetic tree , bacteria , sewage treatment , genetics , gene , physiology , engineering , waste management
Summary A novel coccobacilli group found previously in enhanced biological phosphorus removal (EBPR) systems was further revealed to have a high degree of diversity and distribution in various activated sludge systems. Phylogenetic analysis based on 14 existing and 18 newly retrieved 16S rRNA sequences revealed that these sequences formed a novel cohesive cluster with seven subgroups in the γ‐ Proteobacteria . Fluorescence in situ hybridization with a set of probes designed specifically targeting the novel group at different hierarchical levels showed that the novel group with a coccoid (2–4 µm) to occasionally long‐rod (up to 20 µm) shape widely distributed and in some cases predominated in sludge samples taken from nine lab‐ and full‐scale EBPR systems (10–50% of total cells) and four conventional activated sludge systems (1–10%). Variation of predominance was also observed among those subgroups in systems showing deteriorated or effective EBPR activity.