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Alkane hydroxylase homologues in Gram‐positive strains
Author(s) -
Van Beilen Jan B.,
Smits Theo H. M.,
Whyte Lyle G.,
Schorcht Susanne,
Röthlisberger Martina,
Plaggemeier Thorsten,
Engesser KarlHeinrich,
Witholt Bernard
Publication year - 2002
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1046/j.1462-2920.2002.00355.x
Subject(s) - alkb , biology , pseudomonas putida , microbiology and biotechnology , polymerase chain reaction , gram , gene , pseudomonas , alkane , bacteria , genetics , biochemistry , escherichia coli , catalysis
Summary We isolated Gram‐positive alkane‐degraders from soil and a tricking‐bed reactor, and show using polymerase chain reaction (PCR) with degenerate alkane hydroxylase primers and Southern blots that most Rhodococcus isolates contain three to five quite divergent homologues of the Pseudomonas putida GPo1 alkB gene. Two Mycobacterium isolates each contain one homologue, however there is no evidence for the presence of alkB homologues in the remaining strains.