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Seasonal dynamics of particle‐associated and free‐living marine Proteobacteria in a salt marsh tidal creek as determined using fluorescence in situ hybridization
Author(s) -
Dang Hongyue,
Lovell Charles R.
Publication year - 2002
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1046/j.1462-2920.2002.00295.x
Subject(s) - rhodobacter , biology , proteobacteria , salt marsh , bacteria , fluorescence in situ hybridization , bacterioplankton , marine bacteriophage , phytoplankton , botany , ecology , 16s ribosomal rna , biochemistry , genetics , nutrient , gene , mutant , chromosome
Summary The seasonal distributions of salt marsh free‐living and particle‐associated bacteria belonging to three subdivisions of the Proteobacteria were determined by fluorescence in situ hybridization (FISH) and confocal laser scanning microscopy (CLSM). More than 66% (median = 78%) of total bacterial cells that were stainable with the fluorescent DNA stain Yo‐Pro‐1 were also detected using the bacterial probe EUB338. The α ‐ Proteobacteria , especially those from the marine Rhodobacter group, were abundant on suspended particles and as free‐living cells all year round. The marine Rhodobacter group constituted more than 25% of the particle‐associated bacteria and more than 18% of the free‐living bacteria. Probes specific for three subgroups within the marine Rhodobacter group detected more than 49% of the total marine Rhodobacter group cells. These subgroups displayed different seasonal dynamics. The marine Rhodobacter group is clearly a widespread, diverse and important bacterial lineage in bacterioplankton and particle‐associated assemblages in south‐eastern United States salt marshes at all times of the year.