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Analysis of the Pseudomonas aeruginosa oprD gene from clinical and environmental isolates
Author(s) -
Pirnay JeanPaul,
Vos Daniel De,
Mossialos Dimitris,
Vanderkelen Alain,
Cornelis Pierre,
Zizi Martin
Publication year - 2002
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1046/j.1462-2920.2002.00281.x
Subject(s) - biology , pseudomonas aeruginosa , gene , genetics , horizontal gene transfer , genome , porin , strain (injury) , whole genome sequencing , microbiology and biotechnology , bacteria , escherichia coli , anatomy , bacterial outer membrane
Summary Genomes are constantly evolving. Our report highlights the wide mutational diversity of clinical as well as environmental isolates, compared with the laboratory strain(s), through the systematic genetic analysis of a chromosomal porin gene ( oprD ) in relation to a specific antibiotic resistance. Mutational inactivation of the oprD gene is associated with carbapenem resistance in Pseudomonas aeruginosa . The sequence of the oprD gene of 55 Pseudomonas aeruginosa natural isolates obtained from across the world – from sources as diverse as patients and rhizospheres – was analysed. A microscale mosaic structure for this gene – resulting from multiple intra‐ and possibly interspecies recombinational events – is reported. An array of independent and seemingly fast‐occurring defective oprD mutations were found, none of which had been described before. A burn wound isolate demonstrated unusually high overall sequence variability typical of mutator strains. We also present evidence for the existence of OprD homologues in other fluorescent pseudomonads.