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Genetically engineered Pseudomonas : a factory of new bioplastics with broad applications
Author(s) -
Olivera Elías R.,
Carnicero David,
Jodra Ruth,
Miñambres Baltasar,
García Belén,
Abraham Gustavo A.,
Gallardo Alberto,
Román Julio San,
García José L.,
Naharro Germán,
Luengo José M.
Publication year - 2001
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1046/j.1462-2920.2001.00224.x
Subject(s) - pseudomonas putida , biology , bioplastic , biochemistry , catabolism , polyhydroxybutyrate , genetically engineered , phenylacetic acid , cinnamic acid , mutant , polyhydroxyalkanoates , fermentation , pseudomonas , monomer , bacteria , polymer , gene , metabolism , chemistry , organic chemistry , genetics , ecology
Summary New bioplastics containing aromatic or mixtures of aliphatic and aromatic monomers have been obtained using genetically engineered strains of Pseudomonas putida . The mutation (–) or deletion (Δ) of some of the genes involved in the β‐oxidation pathway ( fad A − , fad B − Δ fad A or Δ fad BA mutants) elicits a strong intracellular accumulation of unusual homo‐ or co‐polymers that dramatically alter the morphology of these bacteria, as more than 90% of the cytoplasm is occupied by these macromolecules. The introduction of a blockade in the β‐oxidation pathway, or in other related catabolic routes, has allowed the synthesis of polymers other than those accumulated in the wild type (with regard to both monomer size and relative percentage), the accumulation of certain intermediates that are rapidly catabolized in the wild type and the accumulation in the culture broths of end catabolites that, as in the case of phenylacetic acid, phenylbutyric acid, trans ‐cinnamic acid or their derivatives, have important medical or pharmaceutical applications (antitumoral, analgesic, radiopotentiators, chemopreventive or antihelmintic). Furthermore, using one of these polyesters (poly 3‐hydroxy‐6‐phenylhexanoate), we obtained polymeric microspheres that could be used as drug vehicles.