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Anaerobic degradation of naphthalene by a pure culture of a novel type of marine sulphate‐reducing bacterium
Author(s) -
Alexander Galushko,
Dror Minz,
Schink,
Widdel
Publication year - 1999
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1046/j.1462-2920.1999.00051.x
Subject(s) - anoxic waters , naphthalene , electron acceptor , bacteria , biology , enrichment culture , oxidizing agent , sulfate reducing bacteria , incubation , sphingomonas , substrate (aquarium) , microbiology and biotechnology , nuclear chemistry , 16s ribosomal rna , food science , environmental chemistry , biochemistry , organic chemistry , chemistry , ecology , genetics
Incubation of marine sediment in anoxic, sulphate‐rich medium in the presence of naphthalene resulted in the enrichment of sulphate‐reducing bacteria. Pure cultures with short, oval cells (1.3 by 1.3–1.9 μm) were isolated that grew with naphthalene as the only organic carbon source and electron donor for sulphate reduction to sulphide. One strain, NaphS2, was characterized. It affiliated with completely oxidizing sulphate‐reducing bacteria of the δ‐subclass of the Proteobacteria, as revealed by 16S rRNA sequence analysis. 2‐Naphthoate, benzoate, pyruvate and acetate were used in addition to naphthalene. Quantification of substrate consumption, sulphide formation and formed cell mass revealed that naphthalene was completely oxidized with sulphate as the electron acceptor.