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Molecular evidence for shifts in polysaccharide composition associated with adaptation of soybean Bradyrhizobium strains to the Brazilian Cerrado soils
Author(s) -
Da Costa Coutinho Heitor Luiz,
Kay Hazel E.,
Manfio Gilson Paulo,
Neves Maria Cristina Prata,
Ribeiro José Roberto A.,
Rumjanek Norma G.,
Beringer John E.
Publication year - 1999
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1046/j.1462-2920.1999.00047.x
Subject(s) - microbial inoculant , biology , bradyrhizobium , polysaccharide , rapd , strain (injury) , adaptation (eye) , soil water , nitrogen fixation , genotype , inoculation , botany , microbiology and biotechnology , bacteria , horticulture , genetics , gene , genetic diversity , biochemistry , ecology , population , demography , anatomy , neuroscience , sociology
Pyrolysis mass spectrometry (PyMS) and DNA fingerprinting (RAPD and RSα hybridization) were used to characterize soybean inoculant strains and root nodule isolates of bradyrhizobia from the Brazilian Cerrado soils. Most isolates were shown to be derived from the inoculant strains on the basis of genotype comparisons by DNA fingerprinting. Phenotypic analysis (using PyMS) of the strains and separately of the polysaccharides derived from them showed that the nodule isolates differed from the parental strains, suggesting adaptation to the Cerrado soil environment. The extent of the differences between the derivatives and inoculant strains was similar for comparisons made on the basis of whole‐cell preparations or from the isolated polysaccharides, indicating that the adaptation was caused by changes in the composition of the polysaccharides produced.